Measurement
UV Sensor

Part:BBa_K154000

Designed by: Mary Jane Stine   Group: iGEM08_Purdue   (2008-10-21)

recA promoter (J22106) + LacZ gene (I732017): DNA damage sensor

This part combines the promoter of the recA gene (an SOS promoter) and the full LacZ gene, with Amp resistance from backbone plasmid pSB1A2. Original part numbers are J22106 (recA) and I732017 (lacZ). The promoter is in front of the LacZ gene, and therefore signals transcription of LacZ when the SOS DNA repair mechanism is triggered by massive DNA damage. LacZ encodes for beta-galactosidase, which cleaves lactose and its homolog X-gal. X-gal is cleaved into galactose and an insoluble blue dye. By plating on an X-gal plate, a bacterium with this plasmid should turn blue when exposed to extensive amounts of DNA damage.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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